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KMID : 0379120120400020078
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Korean Journal of Mycology
2012 Volume.40 No. 2 p.78 ~ p.85
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Genetic Divesity Analysis of Fungal Species by Universal Rice Primer (URP)-PCR
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Kang Hee-Wan
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Abstract
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URP primers that were derived from repetitive DNA sequence of rice weedy rice have been applied for producing PCR polymorphisms in different fungal species. URP-PCR protocol employed stringent PCR with high annealing temperature over 55 oC throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, Each single URP primer produced characteristic fingerprints from diverse genomes of different fungal species, indicating its universal applicability. URP-PCR has been accessed for applicability to various fungal species with 33 genus, 142 species and 1,489 isolates. Numerous related papers have demonstrated that URP-PCR profiles of fungal species are very useful for identifying fungal species at intra and inter species levels. The results were reviewed in this paper.
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KEYWORD
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Fungal species, Genetic diversity, URP-PCR
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